As a matter of fact, human anatomy is a complex thing which has made researchers and scientists undertake many different research and observations trying to understand it. Actually, the body of an individual is built up by a structure called cell. These are minute structures which are found in billions which come up together to form a person. They are the life basic units. When you want to have a better understanding of how they look and work, then live cell microscopy is the way to follow.
This imaging method has been accessible for many years now and has been extended to many different fields. It involves viewing of these structural units under a time-lapse microscope in order to study their cellular structure. Since its pioneering, several methods of such imaging have been developed which allows these units to be studied in great detail. This enhances the knowledge on functions of cellular structures and tissues.
In order for great results to be obtained, the cell under observation should be well maintained. The temperature of the environment should be kept stable. Stability is maintained at 37 degrees for mammalian cellular structures. It is important to use a large microscope as its enclosures offer great temperature stability. The osmolarity of the environment should be avoided. It is caused by evaporation of medium and it is therefore important that the air around the sample is humidified. The mammalian cells require a bicarbonate-based culture media to maintain physiological pH. This will allow capturing of good quality images.
Live-cell fluorescent imaging is able to assist in the creation of high-quality images by using fluorescent proteins to stain them. This allows the images obtained to be multicolored. These techniques are aimed at reducing the reflected light commonly known as the incident light by using fluorescent light. This reduces the photo-toxicity or photo-bleaching as a result of reduced incident light and lack of ultraviolet light as well.
So as to have a clear live-cell imagery, you have to make sure the light you are using is a fluorescent one and minimize any cases of incident light which may contain phototoxic characteristics. Actually, most structures have been functioning in light exposure it is therefore important to reduce this light when making imageries applications. The microscope should be the object to collect light but not exposing the specimen to the light.
This study involves maintaining the balance between obtaining images that are of high quality while also maintaining the cellular health of these structural units. These units are prone to photodamage, especially when there is a presence of fluorophores. Even in the absence of fluorophores, mammalian cellular structures are still sensitive to ultraviolet light.
In this type of research, the imagery is maintained in the most quality manner and cellular health is also maintained. Therefore, this is an experiment that involves balancing both the living aspect of the structure as well as the functionality of the device. These structures are adversely affected fluorophores, UV light and other photo related damages.
Such an imaging system provides many benefits including the detailed assessment of the cell organization of cell structure as well as dynamic processes. The only downside is the damage to the living cell under observation.
This imaging method has been accessible for many years now and has been extended to many different fields. It involves viewing of these structural units under a time-lapse microscope in order to study their cellular structure. Since its pioneering, several methods of such imaging have been developed which allows these units to be studied in great detail. This enhances the knowledge on functions of cellular structures and tissues.
In order for great results to be obtained, the cell under observation should be well maintained. The temperature of the environment should be kept stable. Stability is maintained at 37 degrees for mammalian cellular structures. It is important to use a large microscope as its enclosures offer great temperature stability. The osmolarity of the environment should be avoided. It is caused by evaporation of medium and it is therefore important that the air around the sample is humidified. The mammalian cells require a bicarbonate-based culture media to maintain physiological pH. This will allow capturing of good quality images.
Live-cell fluorescent imaging is able to assist in the creation of high-quality images by using fluorescent proteins to stain them. This allows the images obtained to be multicolored. These techniques are aimed at reducing the reflected light commonly known as the incident light by using fluorescent light. This reduces the photo-toxicity or photo-bleaching as a result of reduced incident light and lack of ultraviolet light as well.
So as to have a clear live-cell imagery, you have to make sure the light you are using is a fluorescent one and minimize any cases of incident light which may contain phototoxic characteristics. Actually, most structures have been functioning in light exposure it is therefore important to reduce this light when making imageries applications. The microscope should be the object to collect light but not exposing the specimen to the light.
This study involves maintaining the balance between obtaining images that are of high quality while also maintaining the cellular health of these structural units. These units are prone to photodamage, especially when there is a presence of fluorophores. Even in the absence of fluorophores, mammalian cellular structures are still sensitive to ultraviolet light.
In this type of research, the imagery is maintained in the most quality manner and cellular health is also maintained. Therefore, this is an experiment that involves balancing both the living aspect of the structure as well as the functionality of the device. These structures are adversely affected fluorophores, UV light and other photo related damages.
Such an imaging system provides many benefits including the detailed assessment of the cell organization of cell structure as well as dynamic processes. The only downside is the damage to the living cell under observation.
About the Author:
When you are searching for information about live cell microscopy, come to our web pages online today. More details are available at http://www.invivoscientific.com/about-us now.
No comments:
Post a Comment